A new antibody for the study of human CD157 expression and function

Abstract CD157 is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. In the human hematopoietic system, prevalently expressed by cells of myelomonocytic lineage, interacts with extracellular matrix components regulates leukocyte diapedesis via integrin-mediated signaling. implicated in pathophysiology several neurological disorders. Although it lacks a cytoplasmatic domain, able to transduce intracellular signals establishing functional structural crosstalk β1 (CD29) β2 (CD18) integrins. ligation causes integrin– dependent cytoskeletal remodeling increase F-actin content. Activation downstream signaling pathways leads cell polarization, migrating rear whereas localized at opposite pole. However, molecular cellular functions still remain poorly understood. Here, we describe generation monoclonal antibody specific for (clone W21007F) can be used flow cytometry, immunofluorescence blocking mediated adhesion monocytes fibronectin. Treatment pro-monocytic, myeloid leukemia line U937 clone W21007F induces polarization marked filamentous actin, clustering uropod. The majority F-Actin fibers are located end CD157, co-localizes integrin beta-1 distinct membrane domains. reference SY/11B5 isotype control do not show this effect. conclusion, anti-human valuable tool study function expression cells.